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This test is specific for the p.V600E mutation with a reported sensitivity of ≥5% mutated alleles in a background of wildtype alleles [ 42].
Molecular clones of vaccine candidates were constructed by inserting either three independently attenuating mutations or a PE2 cleavage-signal mutation with a second-site resuscitating mutation into full-length cDNA clones.
It uses mutation with a higher-than-usual mutation probability to increase the exploration speed and crossover with the parent to repair losses incurred by the more aggressive mutation.
For mutation, with a small probability, flip a random bit in the offsprings' bit streams.
Taken together, these data demonstrate association of the CCR5 Delta 32 mutation with a polymorphism in an as yet unknown gene that is responsible for the ability to down-regulate the expression of high m.w.
Secondly, genetic complementation of the fliC mutation with a copy of wild-type fliC gene restored resistance to SP-A-mediated membrane permeabilization.
Although minor changes in TGR5 expression and plasma membrane localization were noted for several mutations, the premature stop codon Q296X was the only mutation with a major impact.
The yda-1 allele obtained from ABRC (CS6392) also segregated for a second (unlinked) emb mutation with a white seed phenotype.
However, this arrest is abolished in the cdc13 radoubleble mutants that combine the cdc13 mutation with a mutation in the DNA damage checkpoint.
Each chromosome also undergoes mutation with a fixed probability μm.
The BOADICEA model predicts that she carries a BRCA1 mutation with a probability of 40.9% and a BRCA2 mutation with a probability of 1.3%.
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