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Exact(25)
In this study, either three or four iPSC lines from healthy controls and two lines from patients carrying the SOD1D90A mutation were used.
Crossover and mutation were used to generate individuals that represented new potential solutions.
In order to prevent the solution set from falling into local optimal, crossover and mutation were used to generate populations that represented new sets of solutions.
Transgenic male mice B6SJL-TgN (SOD1-G93A) 1GUR (G93A; obtained from Jackson Laboratories, Bar Harbor, MA, USA), over-expressing human SOD1, carrying the Gly93→Ala mutation, were used.
In this method two complimentary oligonucleotides containing the desired mutation were used to prime the PCR on a pET MtbDGC plasmid DNA template.
Both strains, Lox2 (without mutation) and Lox2ΔG (with mutation) were used in this study thus providing a R-IVET system with two different levels of sensitivity.
Similar(35)
For double (or triple) mutations, the mutated vector for the first (or second) mutation was used as template.
Two types of mutation are used, one random and the other 'intelligent' or beneficial.
In next-generation sequencing by Mi-seq (illumina, Japan), genomic DNA including the mutation was used to amplify the region including gRNA2 and gRNA3 target sequences by PCR.
Two genetic operations, which are arithmetic crossover and residual mutation, are used for GRS of the batch filter based on PF PFF batch filter).
Similar to (A), each N-terminal mutation was combined with a mutation I70A at a mid-tunnel region, which impairs the arrest partially (this mutation was used as it did not affect the electrophoretic mobility of MifM).
More suggestions(15)
mutation were treated
mutation were identified
mutation were grouped
mutation were allowed
mutation were included
mutation were labelled
mutation were studied
mutation were determined
mutation were excluded
mutation were classified
mutation were detected
mutation were rescued
mutation were generated
mutation were amplified
mutation were proposed
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