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Transgenic mice expressing the murine G86R SOD1 mutation were obtained in our animal facility and genotyped as described [9].
Leaner heterozygous mice without the Os mutation were obtained by crossing Os +/+ Cacna1atg−la mice with C57BL/6 mice, and C57BL/6 mice were used as controls.
The pedigrees of the 14 families with the germline mutation were obtained together with the history of cancer reported by both sides of the families; ipsilateral and contralateral to the parental side segregating the R337H mutation (Table 1).
NPC1I1061T homozygous fibroblasts GM18453, GM17909 and the heterozygous GM03123 fibroblast cell line containing the I1061T mutation were obtained from Coriell cell repositories (Camden, NJ). and NPC1I1061T homozygous fibroblasts 83.16 and 59413 were obtained from Dr. John O'Brien (Mayo Clinic, Rochester, MN).
Seeds segregating for the abi3-17 mutation were obtained from the Arabidopsis Biological Resource Center.
Fibroblasts from two other patients with the N279K mutation were obtained from the NINDS Coriell Biorepository (https://www.coriell.org/).org/
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A PCR product containing the epe1H297A point mutation was obtained by amplification of the mutated epe1 gateway entry plasmid.
The major structural changes in the subunit structure caused by the S228P mutation was obtained by tracing the structural fluctuations during the 20 ns simulations (Fig. S11A).
Approval for analysis of these samples for the FOXL2 mutation was obtained through the British Columbia Cancer Agency's research ethics board.
T451 to Ala mutation was introduced using the attB2ABF3TA_R primer and S126A mutation was obtained as described in the previous section.
A fragment of LHP1 containing the RNP1.2 mutation was obtained by digestion of pRNP1.2-GFP with EcoRI and transformed into LHP1 CORE-PrAA.
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mutation were ascertained
mutation were generated
relocation were obtained
mutation were available
mutation were treated
mutation were assigned
mutation were identified
mutation were undertaken
mutation were grouped
mutation was obtained
mutation were labelled
mutation were included
mutation were classified
mutation were detected
mutation were amplified
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