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Changes in expression levels and mutation were measured relative to matched normal samples from the same patient.
Samples with and without the T26E active site mutation were measured, at different temperatures (30, 25, 20, 15, and 10 °C), and the data were then subjected to Arrhenius analysis.
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Importantly, although Kras is a single oncogene, several studies show that Kras codon 12 GAT mutation is amplified during carcinogenesis, even when the mutational specificity of the mutagen is other than the G to A mutation being measured.
On the other hand, robustness to mutation is measured from the fitness distribution over individuals with different genotypes.
mtDNA mutation was measured by single molecule PCR.
The structure maintenance through covarying mutation is measured using the mean mutual information of stems which neutrality encompasses as the effect of single mutations on the structure.
Then, the effectiveness of the impact extraction, as well as grounding to the correct mutation is measured on literature describing enzymes.
The frequency of this mutation was measured at six time points based on 10 sequences at each time point (9 sequences at the second time point).
Second, the effectiveness of the impact extraction, as well as grounding to the correct mutation, is measured on literature describing enzymes.
DNA adducts and mutations were measured in caput and cauda spermatozoa, respectively.
Canavanine-resistant mutations were measured on synthetic complete medium containing 0.004% (or 30 mg/liter) canavanine [40].
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