Sentence examples for mutation were expressed from inspiring English sources

When variants of IN-V5 carrying each mutation were expressed in 293T cells, levels of the Y15A-V5, K186Q-V5 and LL241,242AA-V5 IN variants were significantly lower than that of WT (Figure S1A).

The wild type CDC25A catalytic domain (331 524) and N-terminally Flag tagged CDC25A with the C431S active site mutation were expressed and purified as described above.

Channels containing the R365H mutation were expressed in Xenopus oocytes and their surface density, activation by the metabolic inhibitor azide, and blocking by the sulfonylurea tolbutamide were measured (4).

To further determine the pathophysiology of the distinctive clinical presentation of patients with germline KCNJ5 mutations, enhanced green fluorescent protein (eGFP -tagged KCNJ5-beGFP -taggedquence, the G151R mutation, or the G151E mutation were expressed in the mammalian HEKCNJ5-bearingine to determine cell survival rates over time.

It is therefore of particular interest that apoE−/− mice fed the same percentage cholesterol as the HC diet used in our study had reduced plaque development when the apoE−/− mutation was expressed in a mouse strain that completely lacks expression of arginase II [34].

An example of the latter is shown in Fig. 3, where a human Tau construct containing the FTDP-17-associated V337 M mutation is expressed in all 302 worm neurons via a pan-neuronal C. elegans promoter.

Full-length human Ran containing the Q69L mutation was expressed from a pPROEx vector as an N-terminally His-tagged protein containing a TEV protease site.

Since this is a knock-in mouse model, each mutation is expressed under the endogenous promoter, and therefore phenotypic differences were not due to differences in insertion sites or differential expression levels.

This finding is also supported by a recent publication showing unaltered learning abilities in very young (3 4 months) NEP-deficient mice [12], while it was significantly impaired if human Aβ (containing the Swedish double mutation) was expressed in addition in these knockout mice [13], [31].

The P193A mutation is expressed only in the N-terminally extended ADAR1p150 isoform and increases the effect of other AGS mutations in the same protein.

This has not been previously reported; even in studies in which the identical knock-in mutation was expressed in all tissues, prostates were found to be normal.