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iPSCs from two FTD patients with the 10 + 16 mutation were differentiated into cortical neurons.
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Out of the total 2,783 samples, M haplogroup samples (1751) were differentiated based on their specific coding region mutations.
To explore whether LMNA mutation or WRN deficiency may cause aging-associated defects in endothelial cells (ECs), HGPS-ESCs and WS-ESCs were differentiated into HGPS-ECs and WS-ECs, respectively.
The racecars were differentiated only by decals.
Concerning the high rate of mutations it must be differentiated which mutation is causative in the disease (driver mutation) and which is only a bystander mutation (passenger mutation).
This approach, however, probably tolerates a few germline mutations which cannot be differentiated from somatic mutations.
The relative impact of the different family designs and allele frequencies at the mutation could not be differentiated with these simulations.
Although the differentiation in a complete range of neuronal subtypes has not yet been achieved, this model system is extremely promising as it allows to study patient-derived cells which retain the original disease-causing mutation and can be differentiated in virtually all cell types.
TP53 mutation and allelic deletion of the APC gene are detected more frequently in the intestinal phenotype of GC. 8, 16 In contrast, CDH1 gene mutation is detected in differentiated type GC a showing gastric phenotype.
These mutations so far could only be differentiated by using reverse hybridization line probe assay [32].
However, the importance of these mutations in tumorigenesis becomes clear when hESCs are differentiated into normally telomerase-negative cells.
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