Sentence examples for mutation were created from inspiring English sources

Therefore, primers for each mutation were created and used on IDH1 wt cDNA in pDONR221 (DKFZ clone repository).

The E675A catalytic mutation was created by mutating codon 675 gAa to gCa in pch cloned into pUC18Tmini-Tn7T.

Depending on where the mutation is created, it may lead to cell death or transformation.

To determine the importance of this divergent region, a non-sense mutation was created that removes nine residues.

To verify this hypothesis, a homogenous mutation I12S serving as the control to the I12T mutation was created to examine the hydrogen bonding effect on enzymatic activity.

Utilizing a splice site targeting knock-in approach, a 4 nucleotide mutation was created to convert the 5′ splice site of Col2a1 exon 2 from a weak, non-consensus sequence to a strong, consensus splice site.

D474N mutation was created by PCR as described [25].

The RasV12 point mutation was created by PCR-based mutagenesis of the pMK33FlagHis-Ras1 vector.

Wild type DHFR was cloned in a pCDNA3.1 vector and the 829C→T mutation was created using QuickChange site directed mutagenesis kit (Stratagene, La Jolla, CA) as described previously [22].

A +1 frameshift mutation was created within the GFP encoding region of pMF230 and AKN66 using the Phusion™ site-directed mutagenesis kit (FinnzymesOy).

This mutation was created to induce structural changes in the catalytic domain of the enzyme but without loss of its activity.