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Exact(9)
Colonies containing the mutation were confirmed by PCR amplification of the pyrE gene and surrounding DNA (Fig. 4B).
All cases identified with the R337H mutation were confirmed by sequencing the amplified 240 bp of exon 10.
First, CR6261 neutralisation escape variants of RG-A/Indonesia/5/05 were generated, after extensive passaging, that harboured a fixed mutation (H111L) in the HA2 subunit; viruses with this unique mutation were confirmed to be resistant to CR6261 neutralisation (IC50>100 µg/ml vs. 6.25 µg/ml for wild type).
Proper insertion and presence of the mutation were confirmed by sequencing (Macrogen).
The cases showing a mutation were confirmed in a second experiment.
The resulting plasmids with the mutation were confirmed by sequencing of the entire coding region.
Similar(51)
The absence of the FUS mutation was confirmed by sequencing.
The mutation is confirmed by polymerase chain reaction (PCR) and genomic Southern blot analysis.
The mutation was confirmed by sequencing.
The correct mutation was confirmed by PCR.
The presence of the desired mutation was confirmed by sequencing.
More suggestions(15)
transfer were confirmed
variation were confirmed
transformation were confirmed
mutant were confirmed
mutation were treated
mutation were assigned
mutation were grouped
mutation were identified
mutation were undertaken
mutation were included
mutation were labelled
mutation was confirmed
mutation were classified
mutation were detected
mutation were generated
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