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How exactly did you conduct your study? A. Using a mixture of medical science and anthropology, we arranged mini-family reunions with the centenarians and their 70- and 80-year-old "kids," about half of whom had inherited the genetic mutation we were looking for.
Thus for this mutation we were able to confirm the mutation in the original tissue in a subset of alleles.
By choosing a well-characterized hypomorphic singed mutation, we were able to screen simultaneously for fascin-pathway blockers and enhancers in cells containing small amounts of wild-type fascin protein that could potentially serve as a drug target.
We now show that by performing a second round of sequencing after enrichment for the mutation, we were able to increase the sensitivity and specificity of our assay and apply it to invasive carcinomas without the need for microdissection.
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In the current phase of global mutation we are living in, the passage from complexity to hypercomplexity is determined by a series of factors.
But for a mutation, we are all black.
With 35 independent synonymous mutations, we were also able to examine the mutational spectrum of base substitutions.
"While we expected to find some mutations, we were surprised to find that about 5percentt of the stem cell lines we analyzed had acquired mutations in a tumor-suppressing gene called p53," said Merkle.
By combination of FOB1 gene deletion and suppressor mutations, we were able to construct a novel yeast strain showing increased intracellular RNA content.
By using tandem mutations, we were able to determine sequence selectivity regardless of the context of the amino acid.
We excluded those homozygous mutations that were not unique to DT40 and, taking into account that certain genes contain multiple mutations, we were left with homozygous mutations in 485 genes (Table S4).
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CEO of Professional Science Editing for Scientists @ prosciediting.com