Sentence examples for mutation we used from inspiring English sources

To facilitate detection of the novel mutation, we used restriction analysis of a PCR-amplified fragment of genomic DNA spanning 292 bp including part of exon 25, intron 25, and part of exon 26.

To determine if tumors that arose in C/T790M animals similarly had a secondary somatic activating EGFR mutation on the same allele as the T790M mutation, we used oligonucleotide primers that span the human EGFR kinase domain to perform transgene-specific RT-PCR on mRNA derived from nine lung tumor nodules derived from nine different animals (3 from line 37, 5 from line 8, and 1 from line 22).

To estimate the age of the A111T mutation, we used a molecular clock approach.

Rather than the NorpA mutation, we used GMR-hid to ablate larval photoreceptors.

In the presence of mutation, we used simulation to determine the range of the population size under neutrality.

To map the sc mutation we used the 60 K SNP Illumina iSelect chicken array developed by USDA Chicken GWMAS Consortium, Cobb Vantress, and Hendrix Genetics.

Instead of using the recessive o2 mutation, we use an RNA interference (RNAi) construct directed against both 22- and 19-kDa zeins, but linked to the visible green fluorescent protein (GFP) marker gene.

To gain further insight into the distribution of recurrent mutations we used parsimony to estimate the minimum number of mutational steps at each site, based on the inferred phylogenetic tree.

For the functionally relevant obesity MC4R mutations, we used the previously published mutation screen data (525 trios) [21].

For deletion or insertion types of mutations, we used the average of mA% of the first five different waves from the beginning of mutations (Figure S1).

For each pair of mutations, we used Fisher exact test to compute a p-value for statistically significant covariation, along with a lower-bound estimate for the strength of covariation θ based on the 95% confidence interval.