Sentence examples for mutation we removed from inspiring English sources

In the background of an aac2 ∆ mutation, we removed the chromosomal copy of the FZO1 gene, required for OM fusion, yet provided a plasmid encoding both FZO1 and CYH2, allowing counter-selection by a high concentration of CHX in the presence of a chromosomal cyh2 mutation (Sikorski and Boeke 1991).

For mutations present in dbSNP (which are usually germline mutations), we removed potential germline mutations existing in database of dbSNP132 for the downstream analysis.

Considering the prevalence of TP53 mutation and the possible inaccuracy of TTN mutations, we removed these two genes from M   and performed iMCMC on the remaining genes for which two mutated core modules are identified.

We then applied a series of exclusion steps to identify the causative mutation: (1) we removed variants that were heterozygous in the affected animal; (2) we excluded variants that were homozygous in one of the two parents; (3) we rejected variants that were inconsistent with the Mendelian inheritance model; (4) we excluded variants in the non-coding regions.

To exclude the possibility that the ura+ phenotype of the isolated mutants resulted from mutations within the plasmid, we removed the plasmid from the mutant and then re-transfected pSP1-meiURA4.

Additionally, we removed the mutations that were involved in fusion genes because they do not have a single-mutation position.

To evaluate the performance of the original and transFIC score we used WG2+/1 and WGCGC/nonCGC datasets, and a modification of them in which we removed the mutations that appear within the training set of CHASM (WG2+/1* and WGCGC/nonCGC*).

For this analysis, we removed frameshift mutations and premature stop codons from the coding sequence of degenerate neo-Y genes; next, the coding sequences of neo-sex linked geneo-sex linkeded with homologenessequences of D. pseudoobscura and D. melanogaster using TranslatorX (Abascal et al. 2010).

With the selective pressure of mutation removed, we can also directly observe the impact of the penalty on reverse polymerizing organisms due to spontaneous hydrolysis of nucleotide triphosphates.

The Q65L mutation was removed using site-directed mutagenesis to generate the WT sequence.

The strain was backcrossed to unc-119 (ed3 ); rde-1 (ne219 ) and the rrf-3 mutation was removed.