Sentence examples for mutation we performed from inspiring English sources

To determine whether CHO PR328 cells have this mutation, we performed RT-PCR and restriction digestion with MboII.

To confirm the presence of the tax-4 mutation, we performed a soluble compound chemotaxis assay, as described elsewhere [51].

In an attempt to gain insight into the ancestral origin and spread of the SCA10 mutation, we performed an extensive haplotype study using two closely linked STRs and four intragenic SNPs, in families from Brazil and Mexico.

Given that a higher epidemic potential was associated with the E1-A226V mutation, we performed comparative studies using the 37997 African prototype strain and the LR-OPY1 virus isolated during Reunion Island epidemic that bears a mutated envelope glycoprotein.

To find human populations which might include other carriers of the W1197X mutation, we performed a haplotype-based analysis of the paternal (mutant) allele of subject N1's ACE gene.

For each mutation, we performed two amplifications of the RasGAP SH3 coding sequence from the pEG202 construct, using on the one hand the cognate "sens" oligonucleotide and 5'- GTTGCCandAAATAGCGAGTTTAAACC-3', and on the other hand the cognate "rev" oligonucleotide and 5'-GGAAAGAGTTACTCAAGAACAAGAATTTTCG-3'.

To search for potential cooperating mutations, we performed Ion Torrent deep sequencing of recurrently mutated genes in AML [ 8].

To investigate a structural hypothesis for the effects of these mutations, we performed homology modeling of the pore region of wild-type and mutant KCNQ3 channels, using KvAP as a template.

To isolate such mutations, we performed a forward genetic selection for extragenic suppressors of the sec9-4 temperature-sensitive phenotype [27].

To identify the corresponding genes of the new Stu mutations, we performed snip-SNP mapping (see Materials and Methods) [32].

To confirm the pathogenicity of the newly identified mutations, we performed a series of in silico and expression level studies.