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To test the efficiency of plets1 mutation, we measured the DA content in the plets1 males raised at 25°C (18.7±2 ng/mg mass, s.e.m. N = 5) and at 31°C (8.6±1 ng/mg mass, s.e.m. N = 6) respectively.
For each mutation, we measured the magnitude of the WC KATP current at rest after activation by the metabolic inhibitor azide and inhibition by the SU tolbutamide.
Given a mutation, we measured the degree to which its physicochemical properties deviated from the mean physicochemical preference at the query position.
In order to verify the functional significance of the novel HBG2 g.-109G>T HBG2 g.-109G>Teasured Gγ-globin chain levels, using reverse-phase HPLC.
To confirm this observation in a wild-type background (without the glo-4 mutation), we measured the expression of EGR-1 GFP EGR-1 GFPfluorescence staining with an anti-GFP antibydy.
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To investigate the possible structural alterations caused by the ZF1 mutations, we measured the CD spectrum of ZIC3 ZIC35.
This cutoff of 0.3% was chosen here because it is the percentage of mutations we measured within three amino acids around the active site throughout our database.
As pheochromocytomas and NB originate from neural crest and pheochromocytomas frequently show reduced SDH activity caused by pathogenic SDH mutations, we measured the enzymatic activity of SDH in NB.
As PS1 mutations causing early-onset AD modulate protein expression by disrupting endoplasmic reticulum homeostasis, to determine whether decreased Crif1 levels in Tg6799 and APP/PS1 mouse brains were caused by PS1 mutations, we measured Crif1 levels in the brains of 12-month-old Tg2576 mice that carry only the APP mutation (Supplementary Figure 1c).
To apply a further test to the idea that the conductive conformation of the pore is stabilized by the I148T/L267P and W275S GOF mutations, we measured how pHO changes affected the ion selectivity of the mutants in comparison with wild-type K2P2.1 (TREK-1).
To verify the effect of mutations on deacetylation, we measured the rate of decarbamoylation of the enzymes.
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