Although we did not find any mutations, we discovered a novel polymorphism within the kinase domain (398T/C, which leads to amino acid change I133T, Fig. 2A) in 18 cancer cell lines (Table 1) and 101 primary lung cancer tissue samples (Table 2).
Janni and we did the first large-scale screens, but then all of the mutations we discovered had to be worked up, and that sustained a whole generation of postdocs.
Despite the ongoing importance of purging of new mutations, we discover a trove of >400 natural miRNA sequence variants that include single nucleotide polymorphisms in seed motifs, indels that ablate miRNA functional domains, and origination of new miRNAs by duplication.
The density of mutations that we discovered appears to be only slightly lower than what is obtained for Arabidopsis using the same methodology.
When we combine this with the notion that organisms with different food-utilizing strategies are also affected in different ways by genetic mutations, then we discover a new principle, one in which both the fit and the unfit coexist indefinitely".
These results demonstrate that the virulence defects of the ausA deletion mutant in the Wyatt et al. study were caused by the inadvertent mutation in saeS that we discovered.
We discovered mutations in six of the seven strains and, in all cases, these were missense mutations (Fig. 1; Supplemental Fig. 1).
In a large-scale mutagenesis screen, designed to identify Drosophila mutants with abnormally structured neuromuscular junctions (NMJs), we discovered mutations in Drosophila mical, a conserved gene encoding a multi-domain protein with a N-terminal monooxygenase domain.
Previously, we discovered mutations in TP53 to be associated with resistance to anthracycline therapy in breast cancer [ 25].
