Sentence examples for mutation was transformed from inspiring English sources

For this purpose the pdc2Δ519 mutation was transformed into the commercial EC1118 and native Mab2C wine yeast strains.

Plasmid DNA containing the mutation was transformed into XL10-gold ultracompetent cells.

Finally, the DNA vector containing the R246Q mutation was transformed to XL10-Gold ultracompetent cells.

Following the digestion of the methylated/hemimethylated parental DNA with DpnI, the construct containing the double mutation was transformed into E. coli XL-10 Gold (Agilent).

Our results show that the full loss-of-function ben1-1 mutation was transformed to a partial loss-of-function mutation in the bas1-2 sob7-1 ben1-1 (triple-mutant) background showing enhanced levels of the wild-type−spliced transcript.

Once a selectable secondary phenotype was determined, cells containing the suppressor mutation were transformed with a genomic library in YCp50 and transformants were screened for rescue of the secondary phenotype.

Plasmids containing the proper mutation were transformed in electrocompetent JM109 DE3) cells and stored at −80 °C for long-term storage.

A thyrocyte without BRAF mutation is transformed into a follicular carcinoma cells and is further transformed into an anaplastic carcinoma cell by a TP53 mutation.

Cells with the ura4-D18 mutation were transformed with the linear vector DNA and maintained on minimum medium without uracil to select for Ura+ transformants.

Plasmids containing GPN2 with a D106A and Q110L mutation and GPN3 with a D104A and Q108L mutation were transformed into heterozygous deletion mutant strains followed by sporulation and tetrad dissection.

After temperature cycling and treatment with DpnI to digest the parental DNA template and select for the desired DNA construct, the nicked vector DNA incorporating the mutations was transformed into E. coli.