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The T45A-D46A mutation was transferred from the suicide vector down to the virulence plasmid by conjugation and double cross-over performed using previously reported methodology [65].
Each mutation was transferred to the nuo operon expression vector, pBA400, and the resulting plasmids were used individually to transform the nuo deletion strain BA14.
The ICP4-binding site mutation was transferred into the larger plasmids p0−GFP and p0+GFP by subcloning a Xho I - EcoRI restriction fragment to yield the plasmids p0−4BS− and p0+4BS−.
The Mdr2-KO mice develop liver tumors in about 100% of animals by the age of 16 months on the FVB/N genetic background; initially, this model was generated in the 129/OlaHsd strain, but due to a low fertility of the resulting mutants, the Mdr2-KO mutation was transferred into the FVB/N strain [11], [18].
The hpmKIneo-tk+ mutation was transferred into embryonic stem (ES) cells by homologous recombination (see Figure S1B, left panel, in Supporting Information), and the neo-tk selection cassette was subsequently removed by transient expression of Cre recombinase leaving one loxP site in the non coding region (hpmKIlox).
The mutation was transferred into two genetic backgrounds (C57BL/6 and FVB/NJ).
Similar(51)
Although recurrent mutational events can be a likely cause for some of them, interallelic gene conversion event is the most plausible cause that might have resulted in the same mutation being "transferred" into different genomic contexts.
To identify which mutation or combination of mutations was transferred from M184 to the three transformants to give patAB overexpression, the whole genomes of the transformants were sequenced by Illumina sequencing.
Mutations were transferred by P1 transduction (Miller, 1972).
Of these, only the 23S rRNA and spr1021 mutations were transferred to R6T2 and/or 1974T3 (Table 2).
Of the fifteen mutations identified in the 1974M1 mutant (Table 2), six mutations were transferred into both 1974T3 and R6T2 transformants (Table 2).
More suggestions(15)
mutation was reported
mutation was obtained
mutation was characterized
mutation was known
mutation was confirmed
mutation was genotyped
mutation was determined
mutation was backcrossed
mutation was introduced
mutation was termed
mutation was found
mutation was seen
mutation was named
mutation was identified
mutation was transformed
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