Eventually, through multiple generations and recombination events, the marker and the mutation are separated by a recombination event in some individuals.
For example, our platform was less sensitive for minority variants in which two mutations are separated by less than six bases, and we were unable to detect variants with neighboring mutations (Figure S9 and data not shown).
Different molecular signatures were present in the PTC sub-set: cPTC formed a separate sub-group from fvPTC and, among these PTC subtypes, samples with RAS/ BRAF mutations were separated from samples without mutations.
Those two point mutations were separated from each other in DNA sequence of petB, which was important from a genetic point of view for the planned experiments (this separation was one of the reasons for selection of H198N over other mutations in cytochrome b that are also known to inactivate the Qo site but are closer to G158W in sequence).
Although these clusters of mutations are separated in a linear representation of MEK1, when mapped on to a three-dimensional (3D) model of MEK1 they are brought closer together, forming a mutational hot-spot that is proximal to the NRR and the ATP-binding site and thus is well positioned to influence catalytic activity.
For Western blot analyses, albumin depleted plasma samples (SwellGel Blue Albumin Removal Kit, Pierce, Rockford, IL) and protein samples extracted from cerebellar brain homogenates of FTLD patients with and without GRN mutations, were separated by SDS PAGE using pre-cast 4 20% Tris Glycine gels (Invitrogen, Carlsbad, CA) and transferred to nitrocellulose membranes.
Within lineage E, two haplotypes H10 and H3 occurred in a small sub-lineage that was separated by four mutations from other haplotypes (Fig. 2).
The L0d1,2 branch was separated from their ancestral node by two mutations, 498delC and a back-mutation, 16278C!
a: Given 10,000 independent simulations, outcomes for each mutation pair are separated into one or more regions in parameter space (see Figure 2), in addition, the frequency of protected polymorphisms is given for each region (see Figure 4).
The indica cultivar TNS20 and the japonica cultivar Nipponbare had their own additional mutations and were separated into two distinct haplotypes, H4 and H11, respectively.
