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In these studies, mutation was scored by comparing D310 sequences from tumour and corresponding normal tissue/ blood samples, similar to our work.
To genotype individual larvae in the phenotypic analyses, the st67 mutation was scored in genomic DNA samples via a TaqαI restriction enzyme recognition site introduced by the lesion.
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Animals carrying a daf-7 (e1372 ) mutation were scored only if they were dauer larvae by morphology at the end of the assay period.
Eight cases with non-p.V600E mutation were scored as 1+ and therefore negative in the IHC (p.V600K, p.L597R/S, p.E586K and p.D594N).
The functional significance of identified p53 mutations was scored by using the IARC International Agencyy for Research on Cancer) TP 53 Mutation Database (http://www-p53.iarc.fr/).
Mutations were scored relative to the revised Cambridge Reference Sequence (rCRS)[46].
Mutations were scored relative to the revised Cambridge Reference sequence [77].
Mutations were scored relative to the revised Cambridge reference mtDNA sequence [32].
Nine mutations were scored in exon 3 and six in its flanking regions; four of them are new South American tribe-specific singletons.
Mutations were scored according to the following criteria.
Mutations are scored relative to the rCRS [ 30].
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