Sentence examples for mutation was produced from inspiring English sources

Thus lengthening of lifespan is consistent with GarsC201R genotype, and not genetic background effects (although it is formally possible but highly unlikely the effect is due to a BALB/c wildtype gene closely linked to the Gars locus, as the original mutation was produced on a BALB/c background [25]).

Another use of cloned DNA is in vitro mutagenesis in which a mutation is produced in a segment of cloned DNA.

A taxonomy of primary causes for unresolved mutations was produced.

Various synthetic peptides incorporating the designed mutations were produced and their helical content estimated by circular dichroism.

The expected number of neutral mutations produced per generation is equal to the rate at which mutations are produced, multiplied by the population size.

So long as mutations are produced at a steady rate, then the neutral substitutions that we observe will be produced at a steady rate as well.

Mutations were produced through 15 cycles of PCR, where each cycle consisted of 95°C for 1 minute, 58°C for 30 seconds, and 70 C for 10 minutes.

Mutations were produced by a single-step PCR reaction using the following PCR amplification reaction (50 µL) contained Phusion DNA polymerase GC buffer, 200 µM each of the four deoxynucleoside triphosphates, 2 mM MgCl2, 3% DMSO, 80 ng of template DNA (Ets-C31 pET28a vector), 0.5 µM primers forward and reverse, and 1 µL of Phusion DNA polymerase (Finnzymes, USA, Product # F-530-S).

All mutations were produced by site-directed mutagenesis.

Sequence analysis of selected clones was carried out to confirm that only the desired chimeras or mutations were produced.

Therefore, to estimate the rate at which mutations are produced, a correction for selection needs to be implemented.