The virS genes and upstream promoter regions of the mutated plasmids were sequenced to confirm that only the desired mutation was present.
Lastly, when both position 111 and 122 were mutated in the same clone, ouabain resistance was higher than when a single mutation was present [36].
Furthermore, sequencing across the mutated region of genomic DNA derived from SW480 cells revealed that the expected mutation was present (data not shown)).
In women, however, the mutation was present in roughly the same fraction in both age groups.
In addition, a Pro to Ser mutation was present at position 50.
At least 1 mutation was present in 239 (77%) of 311 tumors.
Appropriate ddNTP was used to block the extension when the mutation was present, while the other three dNTPs enabled elongation of the primer.
A missense mutation was present in all of the fibrous dysplasias.
Allelic deletion of the other p53 allele was seen in 67% of ovarian cancers in which a p53 mutation was present.
Four cases of monostotic fibrous dysplasia, four cases of aggressive fibromatosis involving bone, and one case of osteofibrous dysplasia were studied to determine if a mutation was present in exon 8 of the alpha chain of Gs.
In seven of these sequences the G190A mutation was present.
