Sentence examples for mutation was made from inspiring English sources
The strains are listed in Supplementary Table 2. Plasmids expressing SET2 from its own promoter were obtained from Scott Briggs (Purdue University) and the H199L mutation was made in full-length SET2 by site-directed mutagenesis (Quikchange, Stratagene).
PINK1 cDNA carrying Del 245, L347P or E417G mutation was made by staggered PCR.
The N297Q point mutation was made by using site-directed mutagenesis (Quickchange site-directed mutagenesis II kit, Stratagene).
A E357K mutation was made by site directed mutagenesis and the mutant protein expressed and purified to >95% purity as assessed by SDS-PAGE analysis (data not shown).
The Phe217 to Leu217 mutation was made using the QuikChange II site-directed mutagenesis kit (Stratagene), using human wild-type APH1B cloned into pcDNA3.1 Zeo+ (Invitrogen) as template.
The rseAD11H mutation was made in the pRseAB plasmid and the ptsN H73A and H73A/H12A mutations in the pPtsN plasmid using the QuikChange site-directed mutagenesis kit (Stratagene) and verified by sequencing.
This type of mutation is made because it proposes ideas to the user, which do not exist in the chromosomes produced by the crossover operator.
The probabilities for the tumor and germline data are compared, and a prediction about whether the site harbors a somatic mutation is made [ 7- 11].
Thirty strains, each containing one additional mini-Tn 10 insertion mutation, were made from each of the twelve primary gene disruption strains and from the reference strain.
Detection of PU.1 mutations was made on genomic DNA extracted from bone marrow samples.
When the distinction between the carriers of missense or truncating mutations was made, we noted that the cell lines carrying missense mutations were more radiosensitive than those carrying truncating mutations after treatment.
