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The single cell genotyping not only confirmed the co-occurrence of the PTPRT, CAND1 and DOCK6 mutations in the same AML clone but also revealed a clonal hierarchy, as the PTPRT mutation was likely acquired after the CAND1 and DOCK6 mutations.
Thus, we concluded that the causal mutation was likely a sequence variant that affected the protein code or translation.
Bioinformatic analysis revealed the mutation was likely pathogenic (Table 2).
Consequently, a hairless mutation was likely to have forced human ancestors to become facultative bipeds from partial bipeds.
bThis mutation was likely observed because DNA purified from raw blood contains a mixture of tissue sources, including myeloid lineages.
We then used SIFT (Kumar et al. 2009; sift.jcvi.org) to predict whether a nonsynonymous mutation was likely to be deleterious or tolerated.
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Whether such a mutation is likely anytime soon is unknown.
By contrast, I argue that germline mutation is likely to be a very weak balancing force in evolutionary dynamics.
In addition, since an allele of qSH1 is found only in the temperate japonica subspecies, this natural mutation is likely to have been selected during the domestication of japonica rice [16].
Since the selected sh4 allele was found in all examined landraces and modern cultivars of both indica and japonica rice, the sh4 mutation is likely to have been utilized during the very early stages of the rice domestication process.
Introduction of a longer side chain at position 665 by the L665F mutation is likely to generate hydrophobic interactions with the side chains of some of the above-mentioned residues.
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