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The gene affected by the Alq mutation was isolated using a TAIL-PCR protocol [48].
The 3'-end of spx carrying the DD mutation was isolated from pSN56 digested with BclI and SalI.
The PCR product was digested with HindIII and BclI and the 5'-end of spx containing the G52R mutation was isolated.
The F764L mutation was isolated from a patient with complete AIS and was previously characterized as having an abnormally high ligand dissociation rate [21].
The Yar 71 virus, with the corresponding D67G mutation was isolated from a fatally infected individual (Table 1) and ominously, similar substitutions have also been detected in other TBEV isolates from hospitalised patients with encephalitis (Table 2).
While a broad haplotype carrying numerous SNPs was captured on GWAs in a European-origin sample, the apparent causal mutation was isolated to a smaller haplotype present in persons of African descent [4].
Similar(48)
More than 50 associated genes have been identified so far, but multiple different mutations within these genes can cause the condition, and there is a long way to go before every mutation is isolated.
To isolate the aba2 ga1 double mutant, the selected in the F2 generation GA-deficient mutants were self-fertilized and in the next generation lines homozygous for the aba2 mutation were isolated with the previously described molecular marker [29].
Mixed plaques containing the mutation were identified by MAMA-PCR, and from one of these, individual plaques containing the mutation were isolated on the suppressor strain (see Table 3).
To define role of the receptors in signaling, loss-of-function mutations were initially isolated in four out of the five Arabidopsis ethylene receptors, no mutation being isolated for ERS1 [ 13].
Subsequently, the DNA is transformed into ung+ bacteria, where only the newly synthesized, mutant DNA survives and the primer-introduced mutation is isolated.
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mutation was confirmed
mutation was labeled
mutation was repeated
mutation was genotyped
mutation was created
mutation was backcrossed
mutation was introduced
mutation was expected
mutation was termed
mutation were isolated
mutation was found
mutation was named
mutation was identified
mutation was used
mutation was located
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