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However, prior to gene expression, it is important to analyze the DNA construct to see whether the introduction of the mutation was indeed successful.
These data demonstrated that the ropB point mutation was indeed responsible for the lack of SpeB expression in the clinical isolate 5628.
The A* mutation was indeed designed with this goal in mind.
In the unique previous study on this issue, no BRAF mutation was indeed observed in a small subset of human HCC patients.
These data increase confidence that the BRAF mutation was indeed present in cfDNA and that the tumour results are either false negative due to sampling error or not reflective of the mutation status of the metastatic disease.
Sequencing of the entire region between RPN12a and NPTII did not reveal any additional mutations, confirming that the RPN12a STOP codon-associated G to A mutation was indeed sor1.
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The structure of the human PITX2 homeodomain-DNA complex showed that the p.R113P mutation is indeed likely to affect the DNA-binding function of PITX3 [27].
Through genotyping of four patients and two normal relatives in the RP9 family, we found the H137L mutation in the RP9 gene co-segregated with the disease, strongly indicating that the mutation is indeed the genetic cause of the disease.
First, others' structural studies have suggested that BicD typically attaches to cargos via its C-terminal domain, but our sequencing analysis (Fig. 6A) indicated that the location of the point mutation is indeed as expected, in BicD's very N-terminal domain, far from the C-terminal cargo-attachment region.
If a mutation is indeed a true source-gene mutation, the amino acid it encodes may be reflected in the NANOG proteins of other vertebrates.
Interestingly, the R658C (TTD) mutation is indeed known to be temperature sensitive, and human TFIIH complex stability is sensitive to elevated temperature (Vermeulen et al., 2001).
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