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Directionality of the mutation was impossible to determine in one case because of a 5 bp difference to both possible parental alleles.
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The efficiency of ACE is such that success is assured and, moreover, cannot suffer from false positives as the nature of the pyrE deletion is such that reversion of the mutation is impossible.
While expanded alleles sizes within the range of full mutation were impossible to determine with the methodology employed, the alleles within the premutation range were successfully genotyped with a CGG repeats number varying from 55 to 104.
Even in the case of our gene-driven strategy, X-linked mutations are impossible to obtain because of the breeding protocol which is used [11].
It is important to note that mutation accumulation inference methods only make predictions about mutations that have moderate fitness effects mutations with weak fitness effects are difficult to detect, and neutral mutations are impossible to detect.
At three locations where the chimp and human genomes were almost identical, telltale mutations indicated that it was impossible to get a viable protein from the chimp DNA sequence.
60 Moreover, one might be interested in the proportion of mutations with S around 1, as this class of mutation might be impossible to select out and yet might be damaging to the mean fitness of populations.
Unfortunately, the stomach cancer patients had succumbed to their disease and it was impossible to obtain mutation data from any of them.
Why impossible? A. I was impossible.
Now this was impossible.
Conversation was impossible.
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