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»… if we could modulate β-secretase cleavage towards the β′-site, it would have potential therapeutic benefit…« These interesting results and interpretations are subject to a caveat however, that we cannot be sure that the mutation was genuinely pathogenic.
To ensure that this mutation was genuinely present in the original sample, PCR and sequencing of the gene region around this mutation were performed using the original ClbiNPV DNA.
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We used PCR amplification to amplify this region from four of the C. jejuni isolates testing positive for SSH629-D8, including strain 629, and confirmed that this mutation is genuinely present and not an artefact of the SSH procedure.
To address this issue, we analyzed the corresponding recombinant variant proteins aiming (i) to evaluate whether the mutations are genuinely pathogenic, (ii) to assess the severity of the mutation's impact and (iii) to elucidate the molecular mechanisms of loss-of-function.
Another argument against the redundancy model is that if a protein-coding gene was genuinely redundant then eventually, as it diverged from its functional paralog, it would accumulate an obvious inactivating mutation.
It was genuinely sensational.
I was genuinely remorseful.
Halleck was genuinely bewildered.
He was genuinely afraid.
This week was genuinely fun".
Until she was genuinely sorry.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com