Sentence examples for mutation was generated from inspiring English sources
The point mutation was generated using PCR overlap extension technology with the mutated PCR primers (5′-GTGTTGCGCCTGCGAGGTGGCAgaAgaGAGCCTTCTCTC-3′ for I77,78R; 5′-GTGTTGCGCCTGCGAGGTGcaAgaATTGAGCCTTCTCTC-3′ for G76A/I77R; 5′-GTGTTGCGCCTGCGAGcaGcaATTATTGAGCCTTCTCTC-3′ for G75,76A; 5′-GTGTTGCGCCTGCGAGGTGGCAgaATTGAGCCTTCTCTC-3′ for I77R; and 5′-GTGTTGCGCCTGCGAGGTGcaATTATTGAGCCTTCTCTC-3′ for G78A).
The hha(C18E) mutation was generated in a pET15b expression plasmid35 that produces a His6-tagged form of Hha.
The pAH79 derivative containing the gtsB(A10T) mutation was generated by amplifying the 2-kb gtsB locus using primers 22F-5324097 and 22R-5324097 from DNA isolated from an evolved clone carrying the mutation.
A mutation was generated in the site complementary to the miR-139-5p miR-139-5p miR-139-5pUTR of AMFR or NOTCH1, aseeddicated.
The RasK104R point mutation was generated by PCR-based site-directed mutagenesis of pDNR-Dual-Ras1.
The original Fmr1 knock-out mutation was generated using 129P2 stem cells [12].
iPSC lines harboring the m.3243A>G mtDNA mutation were generated from patient fibroblasts with various levels of heteroplasmy.
Mice with conditional Separase inhibitory phosphosite mutation were generated, genotyped and sex determined as described [30].
A nonsense (NS) and a frameshift (FS) mutation were generated introducing the alterations with two different forward primers.
The mutation is generated by an insertion of the ROSA β-gal gene trap vector (Friedrich and Soriano 1991).
The pWSKDE-KR mutant (K13R K19R K23R K37R K41R K541R mutations) was generated by site-directed mutagenesis with the Quikchange kit (Stratagene).
