Sentence examples for mutation was done from inspiring English sources

Complementation of the sarA mutation was done as previously described [36].

Complementation of the alsSD mutation was done by cloning the region spanning 431 bp upstream and 118 bp downstream of the alsSD operon (primers alsSDproF and alsSD downstream KpnI) into the E. coli/S.

Complementation of the nuc mutation was done by cloning the region spanning 453 bp upstream of 277 bp downstream of the nuc gene (primers nuc comp-F and nuc comp-R) into pLI50.

Screening of five randomly picked P. pastoris transformants per mutation was done in 1000 mL shaking flasks.

Detection of BRAF V600E mutation was done with the Taqman allelic discrimination assay (reagents from Applied Biosystems), which has been described in detail elsewhere (Benlloch et al, 2006).

Mutation was done by deletion of the seed sequence of the predicted miR-128-binding site using the primers as described in Supplementary Table S3.

In stabilizing the interface, mutation is done in the interface for forming an ion pairs between the monomers.

As the state value of the control object is generated, applied evolutionary strategy each sampling time because the learning process of an estimation, selection and mutation is done in real-time.

Then, the swap mutation is done.

The other difference between these two algorithms is how mutation is done.

Third, if the convergence conditions are not satisfied, selection, intersection, and mutation were done to the population.