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TaqMan method of kdr mutation was developed following a previously described method [47].
By analysis of gene expression from 1,800 breast cancers, a gene expression signature associated with PIK3CA mutation was developed (PIK3CA-GS).
To circumvent these molecular targets, vemurafenib, a highly selective inhibitor of BRAF V600E mutation, was developed and have shown clear clinical benefits including significantly better response and higher OS rates compared with commonly utilized standard chemotherapy agents [ 22].
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For each representation, special genetic operators (crossover, mutation) are developed to help GP create new individuals based on parent programs.
The algorithm of particle swarm optimization (PSO) with mutation is developed to optimize the parameters of constructed wavelet LS-SVM regression (W-LSSVR).
Here, a label-free colorimetric isothermal cascade amplification for ultrasensitive and specific detection of K-Ras point mutation is developed based on a double-hairpin molecular beacon (DHMB).
The heuristic searching strategy combining the arithmetical crossover, uniform and non-uniform mutation and deletion/insertion mutation is developed for optimizing the rules base (RB) and tuning the triangular-shaped membership functions to increase the efficiency and accuracy of a fuzzy rule-based system (FRBS).
The modified error-prone PCR method, which achieves higher frequencies of base substitutions and both transition and transversion mutations, was developed using mixtures of triphosphate derivatives of nucleoside analogs [150, 151].
In this study, 'RetinoGenetics', which contains more genes and mutations, was developed to provide full-scale mutation information and extensive annotations for genetic retinal disorders.
Once libraries of tumors that mimic various genetic mutations are developed, clones from genetically distinct tumors can be isolated and co-transplanted into recipient zebrafish to recapitulate intratumoral heterogeneity and animals can be examined for the effects of drug treatment on clonal outgrowth and evolution.
A soybean mutant library with a high mutation density was developed and various mutant phenotypes were observed at a high percentage in comparison with other previous soybean mutant libraries.
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