Exact(3)
Whether this mutation was derived from the cloning procedure or reflects a low-level variant present in the parental vaccine stock is unclear.
Briefly, the mouse factor VII cDNA containing a K341A mutation was derived from a previously constructed Icon plasmid vector pcDNA3.1/mfVII K341A)/hIgG1 Fc (Hu et al, 1999; Hu and Garen, 2000, 2001).
Genomic DNA was also extracted from non-hematopoietic cells of hairs or nails to examine whether the mutation was derived from the germline mutation in familial LGL L cases.
Similar(5)
These frequencies could reflect the immediate effect of the mutation in the female's genotype, or a cumulative (multi-generational) effect of the mutation in the stock from which the TP5 element and the mutation were derived.
VAF clusters correspond to tumor subpopulations, and the cellular fraction, or fraction of tumor cells containing the cluster of somatic mutation, is derived from the VAF of the clusters.
Moreover, GISTs from a same patient with germline mutation are derived from independent clones and show different cytogenetic abnormalities, suggesting that different progression pathways can lead to GIST from polyclonal hyperplasia.
U2OS HIF-1α (344–826 V5) also with N416R, M561A and M568A mutations was derived and cultured using described protocols [ 12].
The number of possible synonymous and non-synonymous mutations was derived by examining the longest annotated coding transcript per gene (transcript length based upon Ensembl Biomart described above).
Related(2)
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