Sentence examples for mutation was constructed from inspiring English sources

Also, a knockout strain carrying an acr::cat insertion mutation was constructed.

The cdk9T212E mutation was constructed and introduced into the genome as described elsewhere for cdk9T212A [20].

A ΔclpK mutation was constructed in K. pneumoniae C132-98 by allelic replacement.

The ecsA::intron mutation was constructed by using the TargeTron system (Sigma-Aldrich) with the vector plasmid pNL9164 according to the instructions of the manufacturer.

To test whether [Fe-S] clusters are critical targets for RNS-dependent killing of B. mallei, a mutation was constructed in the NO-induced, [Fe-S] cluster repair regulator iscR.

15 A TEVSer mutant (C151S mutation) was constructed, and the substitution of the cysteine thiol nucleophile by a serine alcohol was found to reduce activity by four orders of magnitude.

MG1655 containing the rpsAD210Y mutation and WC196LC containing the rpsAD210Y mutation were constructed by phage P1 transduction using the phage P1 obtained from the FitnessOle ycaI deletion strain.

Plasmids containing p.R776G mutation were constructed by inserting a BstXI fragment obtained by PCR amplification of an EGFR segment from a cDNA carrying this mutation into a BstXI-digested pcDNA3-EGFR vector.

For each of the seven positions, 20 forward and reverse oligonucleotides encoding each amino acid mutation were constructed and ordered from IDT. Oligonucleotides containing two mutant codons were used for the LexA Pa A89C/I94C double mutant.

Then, the library of mutations was constructed with that combination of genes SPT15-Mu and TAF23-Mu.

In order to prove that these mutations were responsible for the disease state, each of the three mutations was constructed separately by site-directed mutagenesis of the normal PNP cDNA, and each was transiently expressed in COS cells.