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After recombineering, embryonic stem cells were screened via PCR and sequenced to confirm that the D320K mutation was appropriately introduced into the Nrp1 locus.
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The net change in the number of deleterious mutations was then calculated and the virus's mutation load was appropriately increased or decreased.
An explicit mathematical formulation is not required in implementation and, the parameter values, such as; population size, crossover rate and mutation rate are appropriately chosen to maintain the desired accuracy in the solution.
Their production was appropriately mythic.
It was appropriately challenging.
The atmosphere was appropriately eerie.
The ceremony was appropriately deafening.
Mr. Obama was appropriately humble.
The weather was appropriately British.
Liz was appropriately impressed.
It was appropriately wonderful.
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