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The L110P mutation was always compound heterozygous with E148Q mutation, and there were no patients heterozygous for L110P alone.
The fact that the mutation was always found in addition to the wild type is also to be expected with artefacts due to dideoxy terminator misincorporation.
The T790M mutation was always present together with the original sensitizing EGFR mutation and the appearance of T790M always occurred together with an increase in the amount of the sensitizing EGFR mutation (Fig. 1A).
When there was uncertainty whether a mutation in the 16184 16193 region was the result of a substitution or an in-del, the mutation was always treated as a substitution.
Similar(56)
In a letter to Clerselier, Descartes makes clear that of all of his rules of collision "depend on only a single principle, which is that when two bodies collide, and have in them incompatible modes, there must undoubtedly occur some mutation of these modes to make them compatible, but this mutation is always the least possible" (Descartes 1964 74: V, 185, emphasis added).
This can easily be done by designing primers overlapping the internal BsaI site(s), but containing a single nucleotide mismatch (a silent mutation is always possible) to eliminate the BsaI recognition sequence (Fig 4).
However, any reduction is of no consequence, as the pyrE mutation is always repaired to prototrophy, using the ACE correction vector pMTL-ME6, with restoration of wild-type solvent yields (Additional file 4: Fig. S5).
As a result, the protein encoded by the coding sequences is always the same in the genetic algorithm since crossovers only occur at the boundary of the codon triplets and mutation is always performed with reference to the hash table of synonymous codons for each respective amino acid.
Of the glucocorticoid receptors that have been looked at in different species, five specific mutations are always present and distinguish them from the ancestral receptor.
They suggested revising the section on mutation to more directly confront the misconception that mutations are always adaptive.
NPM1 mutations were always accompanied by additional mutations and NPM1 had the highest VAF in only one case.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com