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A double mutation was achieved using the mutated Cys286 DNA as a template for introducing a mutation at Cys295 to alanine.
Complementation of the mutation was achieved using a 7.6-kb genomic DNA fragment containing the promoter and coding region of GLR3.1.
Complementation of the lrgAB mutation was achieved using a plasmid expressing the lrgAB operon using its native promoter.
The Rev inactivation mutation was achieved by incorporation of a early stop codon in the BamHI site.
When required, sorbitol was added at final concentration 1.2 M. Synchronization of strains carrying the thermosensitive cdc25-22 mutation was achieved by growing the cells at 25°C to the early log phase (OD595 = 0.5) and then shifting the cultures to 37°C for 4 hours.
In contrast, robustness to mutation was achieved for GRNs evolved only under a high noise level σ > σ.
Similar(53)
This phenotypic anticipation is paralleled by increases in the relevant repeat length as it is passed from one generation to the next, with increasing size leading to increasing instability, until a "full expansion" mutation is achieved, generally several generations following the initial appearance of the disorder in the family.
Overcoming the reduction in function of a hypomorphic mutation is achieved through an increase in expression levels, that is, compensation.
Prenatal assessment of heteroplasmy levels of familial mtDNA point mutations was achieved using mutation-specific pyrosequencing assays.
The clinical symptoms, laboratory values, and MEFV gene mutations were achieved from the archives records.
Reproducible and reliable mutation detection was achieved at a concentration of mutant DNA below 5% for 11/12 mutations and ≤3% for 9 mutations.
More suggestions(15)
transformation was achieved
mutation was established
mutation was provided
mutation was described
mutation was detected
mutation was labeled
mutation was confirmed
mutation was repeated
mutation was genotyped
mutation was created
mutation was backcrossed
mutation was expected
mutation was introduced
mutation was termed
mutation was found
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