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The luminescent bacterium P. phosphoreum (T3 mutation) used in this study was bought form Institute of Soil Science, Chinese Academy of Sciences, Nanjing, China.
The D469del mutation, used in this study, is the most common PSACH mutation accounting for approximately 30% of cases [34].
The loss-of-function dsx1 mutation used in this study caused a slightly different degree of intersexuality in XX and XY flies (Fig. 3C, E).
Besides the G12V HRAS mutation used in the transformation experiment to derive the premalignant cell line MCF10AT from MCF10A, the only mutation we detected was PIK3CA H1047R, which is known to be the most common activating mutation in the PIK3CA gene in human cancer [15], [16].
R/A mutation used in this paper may affect overall electrostatic of the pocket and thus bias packing of ions.
The Tex19.1 mutation used in this study is a complete replacement of the Tex19.1 open reading frame (19), back-crossed three times to C57BL/6 mice (Charles River).
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All mutations used in this study maintained on the inbred (C57BL/6) background.
The deletion mutations used in this work were isolated as previously described [15].
Other deficiency stocks and specific mutations used in the screen were also obtained from the Bloomington Drosophila stock center.
(A ) Table showing the mutations used in this figure.
A summary of key mutations used in this study is shown in Table 1.
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