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After ARTP mutation, the spore suspension was transferred onto solid medium.
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Therefore, it seemed likely that the G to A transition in the tih gene was the causative mutation in the spore color mutant fus, and that the transition might lead to a splice defect of the second intron.
We report here direct evidence that spores display enhanced survival relative to vegetative cells in passage through the gut of Drosophila melanogaster, and that mutations specifically affecting the spore wall reduce their survival rate.
We found that the mlh3 Δ mutation can partially suppress the spore viability, sporulation defects, and high frequency of aberrant events observed in mms4 Δ strains (Tables 6 and 8).
At flanking markers cntg-142-11335 and cntg-143-25286, there were two progeny for which spore colour did not match genotype, indicating that the mutation affecting spore colour is located between these two markers that are located 57.8 kb apart.
After exposure in space the survival of and mutation induction in the spores will be analyzed at the DLR, together with parallel samples from the corresponding ground control experiment performed in the laboratory.
The effect of mutations that alter the inner layers of the spore wall on the localization of Spr1-GFP was also examined.
For instance, the Smo mutation (Spore Morphology) causes abnormally shaped conidia, which show a non-polarized shape.
The relevance of this mutation in spore germination negative phenotype is not clear at this time.
These cells differentiate into spores and four different cell types to carry the spore mass aloft.
The Spore demonstration is over.
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