Second, studies exhibit significant heterogeneity.
According to the capillary sequence data, the 21 patients in this study exhibit a total of 30 mutations, including 23 missense, 4 insertion, and 3 deletion mutations (Table S2).
Although the zipper region also exhibits variability, multiple mutation studies have found that it confers interaction preferences and is essential for dimerization (Reddy et al. 1992; Arsura et al. 1995; Orian et al. 2003).
All patients included in this study exhibited multiple resistance mutations at baseline with a median number of mutations of 7 and 3 for RT and PR, respectively.
This study exhibits several limitations.
Findings from large studies have shown that patients with PD have an increased frequency of mutations in GBA and that GBA mutation carriers exhibit diverse parkinsonian phenotypes and Lewy body pathology.
Whole exome sequencing may fill in the diagnostic lacuna of at least 22% of the patients who failed in this study to exhibit mutations in any of the known ARCI genes, indicating the existence of additional loci, such as 2 loci on chromosome 12p11.2-q13.
Firstly, an in vitro study showed that KRAS codon 13 mutations (mainly the p.G13D mutation) exhibited weaker transforming activity than codon 12 mutations [ 15].
Microsatellites interrupted by even a single point mutation exhibit a twofold decrease in their mutation rate when compared to pure AC repeats.
In this study, the positive rates of K-ras mutation did not exhibit any significant difference between the postmenopausal-amenorrheic and the premenopausal groups.
