Exact(1)
A tyrosine serves as the general acid for other GNAT enzymes, but the mutation studies eliminate the two active tyrosine residues, Tyr-64 and Tyr-185, as possibilities.
Similar(59)
Further studies using point mutations that eliminate key residues predicted to interact with EMD 57033 are needed to confirm the binding site.
Previous studies using genetic mutations that eliminate function of all isoforms or UNC-13L demonstrate an essential role of UNC-13L in neurotransmitter release (Richmond et al., 1999).
So how do we decide as a society which mutations to eliminate and which to promote?
Further, mutations that eliminate the microtubule-binding domain of APC result in defective chromosomal segregation [46].
However, the mutations completely eliminate autoubiquitination.
The dominant negative protein has two mutations that eliminate phosphorylation sites, preventing IκB from being phosphorylated.
Consequently, the flow field mutations were eliminated.
Bacman et al also targeted CD; however, a different point mutation (m.14459G>A) was eliminated in their study.
This mutation lies in the N-terminal Eh-1 motif in PRH and our previous studies have shown that this mutation eliminates the interaction between TLE1 and the PRH N-terminal domain [ 7].
10.7554/eLife.03720.005 Figure 2. The Nrp1D320K mutation selectively eliminates VEGF-NRP1 binding in vitro.
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