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While the A63V+R193H double activator combination slowed relaxation beyond that of either mutation studied in isolation, the net effect was not precisely additive on a one to one basis as the sum total of relaxation times for single R193H and A63V myocytes (355+246 = 601 ms, 75% Relaxation) was ∼17% greater than those measured in A63V+R193H dual gene transfer myocytes (493 ms).
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The missense mutations studied in this paper represent all of the currently identified missense HGSNAT mutations.
In Fig. 4C and D all the mutations studied in the EF loop of the bacterial subunit corresponding to ND1 are reported.
Very few of the behavioral mutations studied in C. elegans labs are convincing candidates for a useful setting on the evolutionary control panel.
This is the first report of a COL7A1 mutation study in DEB from Korean patients.
In E. coli lacking MutH, MutS, and MutL proteins, forward mutation studies in the lacI gene showed a 200-fold increase in mutation rate [32], [44], [45].
The latter is also consistent with recent mutation studies in Kv channels [4], [33], as well as in Ca+2 activated channels [34] where its linker was also shown to regulate ionic currents.
(c) Packing perturbation and cysteine mutation studies in S4 suggest that KCNE1 lodges at the inter-VSD S4-S1 interface between two adjacent subunits and thereby affects Kv7.1 sensor functions.
The regulatory effect on chondrocyte differentiation was verified by gene mutation studies in mice.
However, mutation study in E. coli [ 56] suggested that in this particular position the side-chain length is not important, and a positive charge is "simply" required.
Motif mutation studies in rats have indicated that nuclear respiratory factor 1 (NRF-1), NRF-2 and Sp1 promote basal Tfam gene transcription.
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