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Furthermore, a mutation strategy is designed to avoid premature convergence.

The CRISPR Cas9 mutation strategy is schematically shown in Extended Data Fig. 5.

Firstly, a combined mutation strategy is developed by using two mixed mutation strategies with a prescribed probability.

Besides, Gaussian mutation strategy is presented and embedded in the SHPSOS algorithm to reinforce the robustness and avoid premature convergence in the evolution process of candidates.

To effectively avoid the premature convergence and enhance the information dissemination between subpopulations under the work specialization, a stochastic mixed mutation strategy is first proposed by incorporating a cosine perturbation into the probability parameter setting and using two mutation strategies with this probability to balance the exploration and exploitation.

The working of mutation strategy is as follows.

The mutation strategy was based on that introduced by Thomas and coworkers to test the structural roles of selected amino acids in HIV-1 Rev [33].

The two ETS sites and the mutation strategy are depicted in Fig. 6A, B. As shown in Fig. 6C, mutation of either of the ETS1 sites dramatically decreased the CIP2A promoter activity.

Combination of 8 mutation strategies is generated 64 different variants of DPD.

In this paper, a kind of discrete artificial bee colony with composite mutation strategies is presented to compensate the defects of the single mutation scheme that is easy to get into the local best for PFSSP, named CDABC.

Thirdly, the composite mutation strategies are proposed to enable the DABC to solve the permutation flow shop scheduling.