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Mutation statuses obtained by DIA were concordant with those obtained by DNA sequencing, and yields of mutant peptide (mutant peptide/[mutant + wild-type peptides]) exhibited linear correlation with yields of RAS-mutant mRNA.
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Differentiation grade, tumor type determination, MSI status and K-RAS mutation status were obtained for CC, only.
gBRCA mutation status was obtained retrospectively for patients in whom gBRCA testing had been performed before study entry.
P53 mutation status was obtained using the p53 Amplichip test (Roche, Pleasanton, CA, USA) that detects single base pair substitutions and deletions (Baker et al, 2010).
BRCA1-mutation status was obtained from patient records, obtained through our family cancer clinic.
TP53 mutation status was also obtained from the TCGA FIREHOSE pipeline using MutSig method (Lawrence et al., 2013).
This is the first report of an analysis of the EGFR mutation status in DNA obtained from the pleural effusion fluid of a series of NSCLC patients and evaluation of the relationship between the mutation status and the clinical response to gefitinib.
Bivariate analyses (Table 2) indicated that neither age, BRCA1/BRCA2 mutation status, nor whether NAF was obtained during DL were associated with experienced DL discomfort.
We then compared mutation statuses between primary and metastatic tumors.
In phase II, 20 tumour samples were obtained to investigate mutation status of BRAF and the PI3K subunit, P13KCA.
A cohort of the de-identified colorectal cancer tissues with known p53 mutation status and p53 protein levels was obtained from the database of the First Affiliated Hospital of Harbin Medical University (Harbin, China) with an IRB approval (Zheng et al., 2013).
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