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We found that the presence of the NHEJ mechanism markedly shifted the mutation spectrum from small deletions/insertions to large-scale deletions (from 2% to 24%).
h5vc offers the mutation Spectrum function to compute a mutation spectrum from a tally file (Supplementary Fig. S2).
The mutation spectrum from a real FMDV sample obtained from a foot lesion of an infected cow is also included in Figure 4, this sample underwent the same processing as the RT-PCR sample (minus the initial DNA to RNA transcription step).
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Tandem mutation events are common in mutagen-induced mutation spectra from mutagenicity assays.
Using colony probe hybridization to detect a common hot spot deletion, followed by polymerase chain reaction and DNA sequencing, we have generated 10 mutation spectra from three classes of mixtures (i.e., urban air, cigarette smoke condensate, and municipal waste incinerator emissions).
The mutational spectrum from comfrey-treated rats suggests that PAs in the plant are responsible for mutation induction and tumour initiation in rat liver.
The mutation spectrum ranges from single base substitutions to deletions of several exons, although the majority of reported changes are missense mutations.
However, it would take a large number of extra mutations to change the mutation spectrum derived from more than 1.5 million SNVs.
The mutation spectrum ranges from single base substitutions to deletions of several exons or uniparental disomies, 8 9 although the majority of reported changes are missense mutations (http://www.hgdm.cf.ac.uk/ac/index.php).hgdm.cf.ac.uk/ac/index.php
Base substitution biases were calculated using the Fisher exact test to compare with a wild-type mutation spectrum derived from the literature (Lynch et al. 2008; Lang et al. 2013).
The mutation spectrum identified differs from that observed in other countries.
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