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Exact(7)
Depending on the genotype of samples at the mutation site, the nick between the two ligation primers can be sealed in the presence of DNA ligase.
At the second mutation site, the His115 side chain lies alongside Trp34 in a parallel displaced π-stacking interaction [20] (Fig. 2d).
If the OCP and the target matched at the mutation site, the OCP was circularized by ligase.
On the other hand, if the RNA base is positioned opposite the mutation site, the mismatched template should be replicated faithfully.
In contrast to what was previously seen for WT αS, in the vicinity of the mutation site, the monotonic decrease in cross-peak intensities for increasing n is not observed in all cases.
In the absence of presumably catalytic residues around the mutation site, the surface-exposed nature and taxon-restricted conservation of the loop would suggest a possible role for this region in protein interactions.
Similar(53)
In the first PCR, DNA fragments covering the 5' end to the mutation site and the mutation site to the 3' end were amplified separately in two reactions.
Free energy changes were calculated to elucidate the dependence of the differentiation ability on the mutation site in the toehold region.
Then the mutation operator adds a randomly generated number (0, 1) to gene value at the mutation site of the chromosome.
The mutation site and the fidelity of HPV-16 E7 coding sequence were confirmed by DNA sequencing.
The mutation site in the Mut-primer is bold.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com