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Exact(7)
Nonetheless the complex interaction of these markers with many other gene mutations that can modify the final prognostic impact, or the effect of intrinsic mutational characteristics, such as, allelic burden or mutation site on protein function and prognosis, make molecular risk stratification a highly complex process.
The influence of mutation site on relative risk of breast or ovarian cancer has been a controversial issue.
Relative peak amplitudes at the heterozygous mutation site on the chromatograms from non-tumour and tumour DNA were compared.
Due to the location of the mutation site on the protein surface, the orientation of the side chain towards the solvent and the limited interaction with surrounding residues, the effect of the amino acid exchange is probably negligible.
The primer sequences for amplification of the EGFR mutation site on exon 19 and sequencing were 5′-GCATGTGGCACCATCTCA-3′(F), 5′-biotin-AAAAGGTGGGCCTGAGGTT-3′(R), and 5′-ATTCCCGTCGCTATC-3′, respectively.
The primer sequences for amplification of the BRAF V 600 E mutation site on exon 15 were as follows: 5′-biotin-CTTCATAATGCTTGCTCTGATAGG-3′ (F) and 5′-GGCCAAAAATTTAATCAGTGGAA-3′ (R).
Similar(53)
The most common mutation site of KIT is located on exon 11.
The third group of features consisted of resistance mutation sites on HIV sequence [ 64].
Five more mutation sites on the interface of Cln3-Cdk1 complex were suggested by in silico alanine scanning.
Simultaneous two-site replacement was performed in different loops to assess the effects of multiple mutation sites on BtR175-binding affinity.
To find conserved mutations between species, we checked these mutation sites on the O. profundicola X and Y chromosomes, because this species was assumed to have the same SD locus.
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