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Given that the mutation site occurs within the N-terminal DNA-binding domain of IRF-5, we thus adopted bioinformatics analysis.
This drastic mutation site occurs in a solvent accessible site where it forms part of β-strand structure within the catalytic domain.
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We found that the tcm5 mutation site occurred at chloroplast transit peptides.
Only those mutation sites occurring in all sequenced genomes of a maternal lineage were placed at the root.
Low outliers, JeffNH,I28A(0)/ JeffNH,WT 0) < 1, indicating enhanced subnanosecond mobility of apo I28A versus apo WT, were distal from the mutation site and occurred in the linker, the PPIase domain catalytic pocket, and the flexible PPIase loop (H64 R80) "capping" the catalytic pocket.
The simplest method of identification involves calling the human allele that matches the chimpanzee as ancestral; however, this procedure implicitly assumes that no new mutation at this site occurred in either chimpanzees or the lineage leading to the common ancestor of all humans.
Moreover, the effect of mutation on a specific site occurred by certain amino acid substitution can be predicted by using mutation effect predication tools.
Our analysis suggests that mutation hot sites occur in residues of high conservation across mammalian SRSs (Table 5).
Mutations at the IOT5 site occurred around the PAM region.
In contrast to the genomic SSRs, EST-SSRs represent functional markers located in the coding fractions of the genome and changes in EST-SSRs length can cause a phenotypic effect, irrespective of the mutation site, whether it occurs in 5′- or 3′-UnTranslated Regions (UTRs) or in the Open Reading Frames (ORFs) [ 8].
Resistance-conferring mutations within the palivizumab binding site occurred in 8.7% of palivizumab recipients and none of the nonrecipients.
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