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(b) Mutation sequences in transgenic calli transformed with pEgPubi-gRNA2-20b (line #8 in Fig. 2) or pEgP237-gRNA2-20b (line #9 in Fig. 2).
At room temperature, detection probes are hybridized between complementary sequences and single-base mutation sequences in the sequence buffer.
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The complete descriptions of NOTCH1 mutations sequences in HD and PEST domains are shown in Additional file 1: Table S1.
Two of the 19 mutations sequenced in the unc-52 study carried a second site mutation less than 400 bp upstream of the primary mutation.
The BAP1 gene has not yet been examined for point mutation by sequencing in non-ccRCC.
Each mutation was sequenced in reprogrammed clones, and pluripotency was determined by differentiation to each germ layer from embryoid bodies.
ERBB2 hotspot mutations sequenced are listed in Table S1.
We found the highest proportion of SSRs with mutations in flanking sequences in the 3′UTR (60.0%) and the lowest in the 5′UTR (25.8%).
However, this simplicity makes viruses ideal tools for analyzing the effect of mutations in regulatory sequences in the expression of regulated genes and, hence, in fitness.
For the determination of germline mutations we sequenced in addition to the tumor tissues from each patient adjacent not affected normal colonic tissue.
Most mutations were sequenced in both directions using either the same forward or reverse primers as for PCR or an internal primer designed for sequencing.
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