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Genomic sequences required for mutation screening were inferred from the following cDNAs or EST: Genbank accession AF259801 and TIGR tentative consensus accessions TC126316 and TC126421 for eIF4E genes and TIGR tentative consensus accessions TC167837, TC 156946, TC 165028 and TC155154 for eIF4G genes.
The primers used for JAK2 mutation screening were as previously described 11.
The sensitivity parameters for BRCA1 and BRCA2 mutation screening were set as 70%and80%0% respectively.
Process and data management of the mutation screening were carried out as described by Voegele et al. [ 36].
The 768 amplicons that were used in the mutation screening were designed to cover exons of genes of interest.
Individuals who showed negative results in mutation screening were also clinically evaluated to confirm their disease status, clinical results confirmed mutation screening.
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Mutation screening was performed for known FHM-related genes.
The implications for prevention of CF and other rare Mendelian diseases through such surveys of mutation screening are discussed.
Mutation screening was performed by direct DNA sequencing of PCR products.
Mutation screening was performed by direct sequencing of the PCR products.
Furthermore, bcr/abl mutation screening was performed using allele specific oligonucleotide polymerase chain reaction (ASO-PCR) methods previously described [20].
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