Sentence examples for mutation scanning samples from inspiring English sources

For example, for validation of a method for mutation scanning, samples containing as many different mutations as possible should be included in the validation.

Sequencing is used most effectively as a confirmatory method after pre-screening with a mutation scanning technique for samples that ideally have had enrichment of the tumour component before DNA extraction.

HRMA has been used routinely for mutation scanning on patient samples with small fragments of equal size (up to 400 bp) that differ only 1 or a few nucleotides in sequence [ 7].

We therefore evaluated the use of high resolution melting (HRM) as a rapid mutation scanning tool for TP53 in tumour samples.

In mutation scanning methodologies such as SSCP and DHPLC, samples demonstrating variant profiles are selected for confirmatory analysis with mutation identifying methods such as dideoxy sequencing.

Thus instrumentation with greater temperature uniformity across the samples assayed may have an advantage in mutation scanning.

A plate of 96 samples can be melted in 10 min, which is 100 times faster than DHPLC; mutation scanning by HRM avoids the use of solvents, maintenance of pumps and injectors, and the challenges with DNASep column.

In this study, we have shown that SYTO 9 is a suitable dye for mutation scanning with HRM, as it was able to sensitively detect KRAS mutations in cell line controls and in tumour samples.

Improved flexibility of FMCA by using these probes was illustrated in three representative applications of FMCA: mutation scanning, mutation identification and mutation genotyping, all of which achieved improved color-multiplexing with easy probe design and versatile probe combination and all were validated with a large number of real clinical samples.

We used mutation scanning methods.

However, mutation scanning has the advantage of being rapid.