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Therefore, in order to allow maximum sensitivity, mutation scanning needs to be performed on all MUTYH exons.
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For TILLING, a large population is needed for finding useful mutants, so the mutation scanning method has to be high-throughput.
We previously validated mutation scanning for BRCA1 and 2 using high-resolution melting curve analysis (HRMCA).
High-resolution melting allows mutation scanning by detecting all heterozygous changes.
These analytical methods have been applied previously to mutation scanning [34], [35].
We used mutation scanning methods.
However, mutation scanning has the advantage of being rapid.
Direct sequencing or mutation scanning followed by direct sequencing was performed to screen small mutations.
Furthermore, HRM, like any mutation scanning technique, has a mutation detection sensitivity of <100% (16).
Using DHPLC for mutation scanning of all 65 exons of FBN1, initially no mutation was found in case 9.
Our study employed a mutation scanning technology to screen for LKB1 mutations at the cDNA level (Janne et al, 2006).
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