Sentence examples for mutation scanning methods from inspiring English sources
We used mutation scanning methods.
Mutation scanning methods used in our study led to the identification of 19 additional novel non-pathological variants (Supplementary Table S4).
Mutations in BRCA1 and BRCA2 have been detected with a variety of techniques including multiple mutation scanning methods and Sanger sequencing (reviewed in [ 28]).
Other mutation scanning methods such as single-strand conformational polymorphism (SSCP) [ 6], denaturing gradient gel electrophoresis (DGGE) [ 7] and technologies such as pyrosequencing [ 8] and mass spectrometry (MS) [ 9] have advantages and disadvantages regarding sensitivity, throughput, cost and simplicity.
In many cases, particularly for mutation scanning methods, it is necessary to use a qualitative description to distinguish between a single normal state (negative result) and any number of mutated states (positive result).
High-resolution melting curve analysis (hrMCA) has recently been developed as a post-PCR mutation scanning method which enables simple, rapid, cost-effective, and highly sensitive mutation screening of large genes.
In this report, we use TTGE mutation scanning method to study somatic mtDNA mutations in the entire mitochondrial genome of 20 esophageal tumors and their surrounding tissue.
For TILLING, a large population is needed for finding useful mutants, so the mutation scanning method has to be high-throughput.
We have set up a very fast, simple and non-expensive mutation scanning method that identifies the most common MUTYH mutations and known polymorphic variants, as well as rare DNA sequence changes spread in the coding sequence and 5' UTR.
A third mutation scanning method, high resolution melting (HRM), was used to identify tomato lines with EMS-induced mutations in candidate genes regulating fruit quality and drought tolerance [ 3].
We carried out mutation screening of the nine exons and intron/exon boundaries of RAD51C by high resolution melting (HRM) analysis, a rapid closed-tube mutation scanning method with high sensitivity and specificity.
